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N Engl J Med 336:633640 Foncea R, Andersson M, Ketterman A et al (1997) Insulin-like growth factor-I rapidly activates multiple signal transduction pathways in cultured rat cardiac myocytes

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As a disulfide bond can be cleaved and hence reduced by the excess concentration of a thiol (sulfhydryl) like -mercaptoethanol (which gets oxidized in the process), the free thiol on glutathione can act as a reducing agent in the cell